Broad Bean Stain Virus (BBSV) - DAS ELISA                          

Catalog #:  V143
Source Antibody:  Rabbit polyclonal antibody for both capture and detection.
Test Format:  DAS ELISA                                   

Reactivity:  The test reacted with all BBSV isolates tested. Reaction of the ELISA is moderate strong.  Optical Density at 405nm  is in arrange of 0.800 - >2.200 depending on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is moderate high.  The virus can be consistently detected in infected plant tissues diluted  at 1:810 - 1:2430.

Specificity:  There is no cross reaction with healthy plant  tissues such as bean, pea, cowpea or other host plants. Background is low on all of  the negative control wells.

Application:  The test can be used to detect BBSV in infected host plants.

Products: 

Catalog Number
Description
Test Wells
Price
  $US
Ship Weight
(Estimated)
V143-R1
ELISA Reagents, Alkaline Phosphatase
500
320
2 lb (1 kg)
V143-R2
ELISA Reagents, Alkaline Phosphatase
1000
560
3 lb (1.5 kg)
V143-R3
ELISA Reagents, Alkaline Phosphatase
5000
2300
12 lb (6 kg)
V143-R4
ELISA Reagents, Alkaline Phosphatase
100
160
1 lb (0.5 kg)
V143-K1
ELISA Kit, Alkaline Phosphatase
500
480
2 lb (1 kg)
V143-K2
ELISA Kit, Alkaline Phosphatase
1000
800
3 lb (1.5 kg)
V143-C1
Coating Antibody
500
170
2 lb (1 kg)
V143-C2
Coating Antibody
1000
320
2 lb (1 kg)
V143-C3
Coating Antibody
5000
1380
2 lb (1 kg)
V143-D1
Detecting conjugate, Alkaline Phosphatase
500
220
2 lb (1 kg)
V143-D2
Detecting conjugate, Alkaline Phosphatase
1000
380
2 lb (1 kg)
V143-D3
Detecting conjugate, Alkaline Phosphatase
5000
1600
2 lb (1 kg)
V143-P2
ELISA control, positive
18
28
1 lb (0.5 kg)
V143-N1
Negative control
18
28
1 lb (0.5 kg)
M004-1
Buffers for DAS/TAS, alkaline phosphatase
     500
  130
2 lb (1.0 kg)
M004-2
Buffers for DAS/TAS, alkaline phosphatase
    1000 
  220
3 lb (1.5 kg)
M004-3
Buffers for DAS/TAS, alkaline phosphatase
    5000 
   900
6 lb (3.0 kg)

b

DELISA Reagents include Coating Antibody and Enzyme Conjugate and all plates.

 

D

ELISA Kits include Coating Antibody and Enzyme Conjugate, all Buffer sets, Positive/Negative controls and all plates.

 

It is easy and convenient To Place An Order
To know more about the products:  Product Items
To learn how to perform the test:  Instructions for DAS ELISA  In PDF Format
To be familiar with other components: Buffer Sets - PDF; Controls - PDF

Information About the Virus

Name:  Broad Bean Stain Virus
Acronym:  BBSV
Synonyms:  virus de la mosaique de la fève, MF virus, broad bean F1 virus (Gibbs et al., 1968), broad bean Evesham stain virus
Group/Genus:  Comovirus

Vector:  Transmitted by a vector; an insect; Apion vorax, Sitona spp.; Coleoptera.
Transmission: Virus transmitted by mechanical inoculation; transmitted by grafting; transmitted by seed (up to 10% in some Vicia faba cultivars).
Main natural host plants: Vicia faba

Virus Infection:  Symptoms include systemic mottling, necrosis and malformation. Symptoms vary cyclically over a few weeks, or vary seasonally.
Diagnostically susceptible host species and symptoms: Phaseolus vulgaris cv. Tendergreen - systemic mottle; Pisum sativum, Vicia faba - systemic mottle; necrosis.

Geographical distribution: Spreads in the African region and the Eurasian region. Found, but with no evidence of spread, in Australia from imported seed.

References:
1.  Gibbs, A.J. and Smith, H.G. (1970). CMI/AAB Descr. Pl. Viruses No. 29, 3 pp.
2.  Russo, M., Castellano, M.A. and Martelli, G.P. (1982). J. Submicrosc. Cytol. 14: 1