Broad Bean True Mosaic Virus (BBTMV) - DAS ELISA Home Products Testing Services Supplies Contact Us
Catalog #: V270
Source Antibody: A rabbit polyclonal antibody for both capture and
detection.
Test Format: DAS ELISA
Reactivity: The test reacted with all BBTMV isolates tested. Reaction of the ELISA is moderate strong. Optical Density at 405nm is in arrange of 0.400 - >1.700 depending on the virus titer in the samples tested.
Sensitivity: Sensitivity of the ELISA is moderate high. The virus can be consistently detected in infected plant tissues diluted at 1:30 -1:90.
Specificity: There is no cross reaction with healthy plant tissues such as apple or other host plants. Background is low on all of the negative control wells.
Application: The test can be used to detect BBTMV in infected herbaceous host plant tissues.
Products:
Catalog Number |
Description |
Test Wells | Price $USD | Ship
Weight (Estimated) |
V270-R1 |
ELISA Reagents, Alkaline Phosphatase |
500 |
320 |
2 lb (1 kg) |
V270-R2 |
ELISA Reagents, Alkaline Phosphatase |
1000 |
560 |
3 lb (1.5 kg) |
V270-R3 |
ELISA Reagents, Alkaline Phosphatase |
5000 |
2300 |
12 lb (6 kg) |
| V270-R4 | ELISA Reagents, Alkaline Phosphatase | 100 |
160 |
1 lb (0.5 kg) |
| V270-K1 | ELISA Kit, Alkaline Phosphatase | 500 |
480 |
2 lb (1 kg) |
| V270-K2 | ELISA Kit, Alkaline Phosphatase | 1000 |
800 |
3 lb (1.5 kg) |
V270-C1 |
Coating Antibody |
500 |
170 |
2 lb (1 kg) |
V270-C2 |
Coating Antibody |
1000 |
320 |
2 lb (1 kg) |
V270-C3 |
Coating Antibody |
5000 |
1380 |
2 lb (1 kg) |
V270-D1 |
Detecting conjugate, Alkaline Phosphatase |
500 |
220 |
2 lb (1 kg) |
V270-D2 |
Detecting conjugate, Alkaline Phosphatase |
1000 |
380 |
2 lb (1 kg) |
V270-D3 |
Detecting conjugate, Alkaline Phosphatase |
5000 |
1600 |
2 lb (1 kg) |
V270-P2 |
ELISA control, positive |
18 |
28 |
1 lb (0.5 kg) |
V270-N1 |
Negative control |
18 |
28 |
1 lb (0.5 kg) |
| M004-1 | Buffers for DAS/TAS, alkaline phosphatase | 500 | 130 | 2
lb (1.0 kg) |
| M004-2 | Buffers for DAS/TAS, alkaline phosphatase | 1000 |
220 | 3
lb (1.5 kg) |
| M004-3 | Buffers for DAS/TAS, alkaline phosphatase | 5000 |
900 | 6
lb (3.0 kg) |
ELISA Reagents include Coating Antibody and Enzyme Conjugateand all plates.
ELISA Kits include Coating Antibody and Enzyme Conjugate, all Buffer sets, Positive/Negative controls and all plates.
It is easy and convenient To Place
An Order
To know more about the products: Product
Items
To learn how to perform the test: Instructions for DAS
ELISA
To be familiar with other components: Buffer
Sets; Controls
Information About the Virus
Name: Broad Bean True Mosaic Virus
Acronym: BBTMV
Synonyms: Vicia virus 1, Echtes Ackerbohnenmosaikvirus.
Group/Genus: Comovirus
Vector: Transmitted by a vector; an insect; Apion vorax, Sitonia spp. and other weevils; Coleoptera.
Transmission: Virus transmitted by mechanical inoculation; transmitted by seed.
Main natural host plants: Pisum sativum, Vicia faba.
Virus Infection Symptoms: Vicia faba - mild mosaic and malformation, necrosis.
Diagnostically susceptible host species and symptoms:
- Pisum sativum - systemic chlorosis, shoot necrosis.
- Vicia faba - mottle, mosaic and shoot necrosis.
Geographical distribution: Spreads in the African region and the Eurasian region; China, Morocco, and the UK. Found, but with no evidence of spread, in Australia in crops grown from imported seed.
References:
- Blevings, S. and Stace-Smith, R. (1976). J. gen. Virol. 31: 199.
- Cockbain, A.J., Cook, S.M. and Bowen, R. (1975). Ann. appl. Biol. 81: 331.
- Cockbain, A.J., Bowen, R. and Vorra-Urai, S. (1976). Ann. appl. Biol. 84: 321.
- Gibbs, A.J. and Paul, H.L. (1970). CMI/AAB Descr. Pl. Viruses No. 20, 4 pp.
- Gibbs, A.J., Giussani-Belli, G. and Smith, H.G. (1968). Ann. appl. Biol. 61: 99.
- Jones, A.T. (1978). Ann. appl. Biol. 88: 137.
- Quantz, L. (1953). Phytopath. Z. 20: 421.
- Randles, J.W. and Dube, A.J. (1977). Australas. Pl. Path. Soc. Newsl. 6: 37.
- Russo, M., Castellano, M.A. and Martelli, G.P. (1982). J. Submicrosc. Cytol. 14: 149.