Blackeye Cowpea Mosaic Virus (BlCMV) - DAS ELISA

Catalog #:  V011
Source Antibody:
  Rabbit polyclonal antibody for both capture and detection.
Test Format:    DAS ELISA
Reactivity:  Reaction of the ELISA is moderately strong.  Optical Density at 405nm is in a range of 0.700 - 1.750 depending on the virus titer in the samples tested.
Sensitivity:  Sensitivity of the ELISA is relatively high.  The virus can be consistently detected in infected plant tissues diluted at 1:270 - 1:810.
Specificity:  This test system has no cross reaction with healthy plant tissues tested. Background is low on the negative control wells.


<img src="/BICMV.jpg" width="273" height="243" />

Products: 


Catalog Number

Description

Test Wells

Price $USD

Ship Weight
(Estimated)

V011-R1

ELISA Reagents, Alkaline Phosphatase

500

275

2 lb (1 kg)

V011-R2

ELISA Reagents, Alkaline Phosphatase

1000

470

3 lb (1.5 kg)

V011-R3

ELISA Reagents, Alkaline Phosphatase

5000

1860

12 lb (6 kg)

V011-R4

ELISA Reagents, Alkaline Phosphatase

100

105

1 lb (0.5 kg)

V011-K1

ELISA Kit, Alkaline Phosphatase

500

435

2 lb (1 kg)

V011-K2

ELISA Kit, Alkaline Phosphatase

1000

690

3 lb (1.5 kg)

V011-C1

Coating Antibody

500

140

2 lb (1 kg)

V011-C2

Coating Antibody

1000

260

2 lb (1 kg)

V011-C3

Coating Antibody

5000

885

2 lb (1 kg)

V011-D1

Detecting conjugate, Alkaline Phosphatase

500

190

2 lb (1 kg)

V011-D2

Detecting conjugate, Alkaline Phosphatase

1000

315

2 lb (1 kg)

V011-D3

Detecting conjugate, Alkaline Phosphatase

5000

1080

2 lb (1 kg)

V011-P1

ELISA control, Positive

18

20

1 lb (0.5 kg)

V011-N1

Negative control

18

20

1 lb (0.5 kg)

M004-1

Buffers for DAS/TAS, alkaline phosphatase

500

130

2 lb (1.0 kg)

M004-2

Buffers for DAS/TAS, alkaline phosphatase

1000 

230

3 lb (1.5 kg)

M004-3

Buffers for DAS/TAS, alkaline phosphatase

5000 

900

6 lb (3.0 kg)

DELISA Reagents include Coating Antibody and Enzyme Conjugate.

 

D

ELISA Kits include Coating Antibody and Enzyme Conjugate, all Buffer sets, Positive/Negative controls and all plates.

 

 

It is easy and convenient: To Place An Order 
To know more about the products:
  Product Items
To learn how to perform the test:
  Instructions for DAS ELISA
To be familiar with other components: Buffer Sets; Controls
Information About the Virus
Name: Blackeye cowpea Mosaic Virus
Acronym:  BlCMV
Genus: Potyvirus
Synonyms: Bean common mosaic virus - serotype B, bean mosaic virus, bean western
mosaic virus, mungbean mosaic virus.
Main host plants and diseases: Phaseolus vulgaris, Phaseolus coccineus - tolerant cultivars develop mosaic and malformation; sensitive ones show rugosity of lower leaves, mosaic, malformation of leaves and pods.

Transmission: Transmitted by a vector; an insect; Acyrthosiphon pisum, Aphis craccivora, A. fabae, Myzus persicae and other spp.; Aphididae. Transmitted in a non-persistent manner. Virus transmitted by mechanical inoculation, and transmitted by seed or by pollen to the seed

Geographical Distribution: Probably distributed worldwide (in Phaseolus beans wherever they are grown). Spreads in China and the USA.
References:
1. Demski, J.W., Reddy, D.V.R., Wongkaew, S., Iwaki, M., Seleh, N. and Xu, Z. (1988). Phytopathology 78: 631.
2. Fukumoto, F., Thongmeearkom, P., Iwaki, M., Choopanya, D., Sarindu, N., Deema, N. and  Tsuchizaki, T. (1986). Tech. Bull. Trop. Agric. Res. Center, Japan 21: 150.
3. Wongkaew, S. and Dollet, M. (1990). Oleagineux 45: 268.
4. Zeyong, X., Ziling, Y. and Jialing, L. (1983). Plant Dis. 67: 1029

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