Chrysanthemum Stem Necrosis Virus (CSNV) - DAS ELISA Home    Products    Testing Services   Supplies   Contact Us

Catalog #:  V283 ( VS16 )
Source Antibody:
  Rabbit polyclonal antibody for both capture and detection.
Test Format:    DAS ELISA
Reactivity:  Reaction of the ELISA is strong.  Optical Density at 405nm is in a range of 0.600 - 1.800 depending on the virus titer in the samples tested.
Sensitivity:  Sensitivity of the ELISA is high.  The virus can be consistently detected in infected plant tissues diluted at 1:90 - 1:810.
Specificity:  This test system has no cross reaction with healthy plant tissues tested. Background is very low on the negative control wells.
Products: 


Catalog Number

Description

Test Wells

Price USD $

Ship Weight
(Estimated)

V283-R1

ELISA Reagents, Alkaline Phosphatase

500

320

2 lb (1 kg)

V283-R2

ELISA Reagents, Alkaline Phosphatase

1000

560

3 lb (1.5 kg)

V283-R3

ELISA Reagents, Alkaline Phosphatase

5000

2300

12 lb (6 kg)

V283-R4

ELISA Reagents, Alkaline Phosphatase

100

160

1 lb (0.5 kg)

V283-K1

ELISA Kit, Alkaline Phosphatase

500

480

2 lb (1 kg)

V283-K2

ELISA Kit, Alkaline Phosphatase

1000

800

3 lb (1.5 kg)

V283-C1

Coating Antibody

500

170

2 lb (1 kg)

V283-C2

Coating Antibody

1000

320

2 lb (1 kg)

V283-C3

Coating Antibody

5000

1380

2 lb (1 kg)

V283-D1

Detecting conjugate, Alkaline Phosphatase

500

220

2 lb (1 kg)

V283-D2

Detecting conjugate, Alkaline Phosphatase

1000

380

2 lb (1 kg)

V283-D3

Detecting conjugate, Alkaline Phosphatase

5000

1600

2 lb (1 kg)

V283-P2

ELISA control, Positive

18

28

1 lb (0.5 kg)

V283-N1

Negative control

18

28

1 lb (0.5 kg)

M004-1

Buffers for DAS/TAS, alkaline phosphatase

500

130

2 lb (1.0 kg)

M004-2

Buffers for DAS/TAS, alkaline phosphatase

1000 

220

3 lb (1.5 kg)

M004-3

Buffers for DAS/TAS, alkaline phosphatase

5000 

900

6 lb (3.0 kg)

CSNV

 

DELISA Reagents include Coating Antibody and Enzyme Conjugate.

 

D

ELISA Kits include Coating Antibody and Enzyme Conjugate, all Buffer sets, Positive/Negative controls and all plates.

 

It is easy and convenient: To Place An Order 
To know more about the products:
  Product Items
To learn how to perform the test:
  Instructions for DAS ELISA
To be familiar with other components: Buffer Sets; Controls
Information About the Virus
Name: Chrysanthemum Stem Necrosis Virus
Acronym:  CSNV
Genus: Tospovirus
The virus: CSNV is a member of a tentative tospovirus species. Virions isometric, enveloped with 85 nm in diameter, and rounded in profile without a conspicuous capsomere arrangement.
Main host plants and Symptoms: Chrysanthemums and tomatoes are the only reported natural hosts for CSNV.Symptoms are described as mild or severe necrotic streaks on the stem, wilting of leaves and stems, and chlorotic or necrotic spots and rings on some leaves. Necrosis on peduncles and flora receptacles has been described too.
Transmission: CSNV is transmitted and spread in nature by thrips (Thysanoptera) in a persistent manner. Frankliniella occidentalis and Frankliniella schultzei are main vectors of CSNV.
Geographical Distribution: CSNV was first recognized on chrysanthemum in Brazil. Later it was found on tomato, also in Brazil. In Europe, outbreaks have occurred in the Netherlands, Slovenia and the UK.
References:
1. Colariccio A; Eiras M; Chaves ALR; Louren¦ao AL; Melo AMT; Siqueira WJ, (2000). Phytopathologica, 26(2): 252-254.
2. Mumford RA; Jarvis B; Morris J; Blockley A, (2003). Plant Pathology, 52:779.
3. Yoon JY; Choi GS; Choi SK, (2017). Plant Disease, 101(1):264.

It is easy and convenient: To Place An Order 
To know more about the products:
  Product Items
To learn how to perform the test:
  Instructions for DAS ELISA
To be familiar with other components: Buffer Sets; Controls

 


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