Citrus Leaf Rugose Virus (CiLRV) - DAS ELISA Home Products Testing Services Supplies Contact Us
Catalog #: V156
Source Antibody: Rabbit polyclonal antibody for both capture and
detection.
Test Format: DAS ELISA
Reactivity: The test reacted with all CiLRV isolates tested. Reaction of the ELISA is moderate strong. Optical Density at 405nm is in arrange of 0.800 - >2.500 depending on the virus titer in the samples tested.
Sensitivity: Sensitivity of the ELISA is moderate high. The virus can be consistently detected in infected plant tissues diluted at 1:810 - 1:2430.
Specificity: There is no cross reaction with healthy plant tissues such as citrus or other host plants. Background is low on all of the negative control wells.
Application: The test can be used to detect CiLRV in infected host plants.
Products:
|
Catalog Number |
Description |
Test Wells | Price $USD |
Ship
Weight (Estimated) |
|
V156-R1 |
ELISA Reagents, Alkaline Phosphatase |
500 |
275 |
2 lb (1 kg) |
|
V156-R2 |
ELISA Reagents, Alkaline Phosphatase |
1000 |
470 |
3 lb (1.5 kg) |
|
V156-R3 |
ELISA Reagents, Alkaline Phosphatase |
5000 |
1860 |
12 lb (6 kg) |
| V156-R4 | ELISA Reagents, Alkaline Phosphatase |
100 |
105 |
1 lb (0.5 kg) |
| V156-K1 | ELISA Kit, Alkaline Phosphatase |
500 |
435 |
2 lb (1 kg) |
| V156-K2 | ELISA Kit, Alkaline Phosphatase |
1000 |
690 |
3 lb (1.5 kg) |
|
V156-C1 |
Coating Antibody |
500 |
140 |
2 lb (1 kg) |
|
V156-C2 |
Coating Antibody |
1000 |
260 |
2 lb (1 kg) |
|
V156-C3 |
Coating Antibody |
5000 |
885 |
2 lb (1 kg) |
|
V156-D1 |
Detecting conjugate, Alkaline Phosphatase |
500 |
190 |
2 lb (1 kg) |
|
V156-D2 |
Detecting conjugate, Alkaline Phosphatase |
1000 |
315 |
2 lb (1 kg) |
|
V156-D3 |
Detecting conjugate, Alkaline Phosphatase |
5000 |
1080 |
2 lb (1 kg) |
|
V156-P2 |
ELISA control, positive |
18 |
20 |
1 lb (0.5 kg) |
|
V156-N1 |
Negative control |
18 |
20 |
1 lb (0.5 kg) |
| M004-1 | Buffers for DAS/TAS, alkaline phosphatase | 500 | 130 |
2 lb (1.0 kg) |
| M004-2 | Buffers for DAS/TAS, alkaline phosphatase | 1000 | 220 |
3 lb (1.5 kg) |
| M004-3 | Buffers for DAS/TAS, alkaline phosphatase | 5000 | 900 |
6 lb (3.0 kg) |
ELISA Reagents include Coating Antibody and Enzyme Conjugate and all plates.
ELISA Kits include Coating Antibody and Enzyme Conjugate, all Buffer sets, Positive/Negative controls and all plates.
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An Order
To know more about the products: Product
Items
To learn how to perform the test: Instructions for DAS
ELIAS
To be familiar with other components: Buffer
Sets; Controls
Information About the Virus
Name: Citrus Leaf Rugose Virus
Acronym: CiLRV
Synonyms: citrus psorosis virus complex, citrus crinkly leaf ilarvirus
Group/Genus: Ilarvirus
Vector: Transmitted by means not involving a vector.
Transmission: Virus transmitted by mechanical inoculation; transmitted by
grafting; possibly transmitted by seed.
Main natural host plants: Citrus aurantifolia, C. limon, C.
paradisi
Virus Infection: causes flecking of young leaves and malformation of
old leaves (particularly of Eureka lemons), fruit small and lumpy. Symptoms
persist, or vary seasonally.
Diagnostically susceptible host species and symptoms: Citrus limon
Eureka lemon - asteroid local lesions. Citrus sinensis, C. limon, C.
reticulata - crinkling and flecking. Vigna unguiculata - vein
clearing, malformation. Crotalaria spectabilis - systemic chlorotic
veinbanding.
Geographical distribution: Spreads in the USA (California). Found, but with no evidence of spread, in Australia.
References:
1. Attathom, S., Weathers, L.G. and Greer, F.C. (1975). Proc. Am.
Phytopath. Soc. 2: 111.
2. Gonsalves, D. and Garnsey, S.M. (1976). CMI/AAB Descr. Pl.
Viruses No. 164, 4pp.