Ginger Chlorotic Fleck Virus (GCFV) - DAS ELISA                          Home    Products    Testing Services   Supplies   Contact Us

Catalog #:  V191
Source Antibody:  Rabbit polyclonal antibody for both capture and detection.
Test Format:  DAS ELISA                                   

Reactivity:  The test reacted with all GCFV isolates tested. Reaction of the ELISA is moderate strong.  Optical Density at 405nm  is in arrange of 0.800 - 2.700 depending  on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is moderate high.  The virus can be consistently detected in infected plant tissues diluted  at 1:810.

Specificity:  There is no cross reaction with healthy plant  tissues such as rice and other host plants. Background is relative low on all of  the negative control wells.

Application:  The test can be used to detect GCFV in infected host plants.

Products: 

Catalog Number

 Description Test Wells Price $USD Ship Weight
(Estimated)
V191-R1 ELISA Reagents, Alkaline Phosphatase

500

275

 2 lb (1 kg)
V191-R2 ELISA Reagents, Alkaline Phosphatase

1000

470

 3 lb (1.5 kg)
V191-R3 ELISA Reagents, Alkaline Phosphatase

5000

1860

 12 lb (6 kg)
V191-R4 ELISA Reagents, Alkaline Phosphatase

100

105

 1 lb (0.5 kg)
V191-K1 ELISA Kit, Alkaline Phosphatase

500

435

 2 lb (1 kg)
V191-K2 ELISA Kit, Alkaline Phosphatase

1000

690

 3 lb (1.5 kg)
V191-C1 Coating Antibody

500

140

 2 lb (1 kg)
V191-C2 Coating Antibody

1000

260

 2 lb (1 kg)
V191-C3 Coating Antibody

5000

885

 2 lb (1 kg)
V191-D1 Detecting conjugate, Alkaline Phosphatase

500

190

 2 lb (1 kg)
V191-D2 Detecting conjugate, Alkaline Phosphatase

1000

315

 2 lb (1 kg)
V191-D3 Detecting conjugate, Alkaline Phosphatase

5000

1080

 2 lb (1 kg)
V191-P2 ELISA Control, Positive

18

20

 1 lb (0.5 kg)
V191-N1 Negative control

18

20

 1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase 500
130
 2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase 1000 
220
 3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase 5000 
900
 6 lb (3.0 kg)

DELISA Reagents include Coating Antibody and Enzyme Conjugate and all plates.

 

D

ELISA Kits include Coating Antibody and Enzyme Conjugate, all Buffer sets, Positive/Negative controls and all plates.

It is easy and convenient To Place An Order
To know more about the products:  Product Items
To learn how to perform the test:  Instructions for DAS ELISA
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name:  Ginger Chlorotic Fleck Virus
Acronym:  GCFV
Synonyms: 
Group/Genus:  Sobemovirus

Vector: 
Transmission: Virus transmitted by mechanical inoculation.
Main host plants: Zingiber officinale
Virus Infection:  Symptoms persist. Zingiber officinale - chlorotic leaf flecking.

Diagnostically susceptible host species and symptoms: Zingiber officinale - systemic chlorotic flecking.

Geographical distribution: Found, but with no evidence of spread, in Australia, where it was isolated from ginger imported from India, Malaysia, Mauritius and Thailand, but all infected material was destroyed and there was no evidence that it became established in Australia. Its presence has not yet been confirmed in the countries of origin of the infected ginger.

 References:
1.  Thomas, J.E. (1986). Ann. appl. Biol. 108: 43.
2.  Thomas, J.E. (1988). AAB Descr. Pl. Viruses No. 328, 4 pp.

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