Hibiscus Chlorotic Ringspot Virus (HCRSV) - DAS ELISA Home Products Testing Services Supplies Contact Us
Catalog #: V153
Source Antibody: Rabbit polyclonal antibody for both capture and detection.
Test Format: DAS ELISA
Reactivity: The test reacted with all HCRSV isolates tested. Reaction of the ELISA is moderate strong. Optical Density at 405nm is in arrange of 0.800 - >2.600 depending on the virus titer in the samples tested.
Sensitivity: Sensitivity of the ELISA is moderate high. The virus can be consistently detected in infected plant tissues diluted at 1:810.
Specificity: There is no cross reaction with healthy plant tissues such as hibiscus, or other host plants. Background is relative low on all of the negative control wells.
Application: The test can be used to detect HCRSV in infected host plant tissues.
Products:
|
Catalog Number |
Description | Test Wells | Price $USD | Ship Weight (Estimated) |
|
V153-R1 |
ELISA Reagents, Alkaline Phosphatase |
500 |
275 |
2 lb (1 kg) |
|
V153-R2 |
ELISA Reagents, Alkaline Phosphatase |
1000 |
470 |
3 lb (1.5 kg) |
|
V153-R3 |
ELISA Reagents, Alkaline Phosphatase |
5000 |
1860 |
12 lb (6 kg) |
| V153-R4 | ELISA Reagents, Alkaline Phosphatase |
100 |
105 |
1 lb (0.5 kg) |
| V153-K1 | ELISA Kit, Alkaline Phosphatase |
500 |
435 |
2 lb (1 kg) |
| V153-K2 | ELISA Kit, Alkaline Phosphatase |
1000 |
690 |
3 lb (1.5 kg) |
|
V153-C1 |
Coating Antibody |
500 |
140 |
2 lb (1 kg) |
|
V153-C2 |
Coating Antibody |
1000 |
260 |
2 lb (1 kg) |
|
V153-C3 |
Coating Antibody |
5000 |
885 |
2 lb (1 kg) |
|
V153-D1 |
Detecting conjugate, Alkaline Phosphatase |
500 |
190 |
2 lb (1 kg) |
|
V153-D2 |
Detecting conjugate, Alkaline Phosphatase |
1000 |
315 |
2 lb (1 kg) |
|
V153-D3 |
Detecting conjugate, Alkaline Phosphatase |
5000 |
1080 |
2 lb (1 kg) |
|
V153-P2 |
ELISA control, positive |
18 |
20 |
1 lb (0.5 kg) |
|
V153-N1 |
Negative control |
18 |
20 |
1 lb (0.5 kg) |
| M004-1 | Buffers for DAS/TAS, alkaline phosphatase | 500 | 130 |
2 lb (1.0 kg)
|
| M004-2 | Buffers for DAS/TAS, alkaline phosphatase | 1000 | 220 |
3 lb (1.5 kg)
|
| M004-3 | Buffers for DAS/TAS, alkaline phosphatase | 5000 | 900 |
6 lb (3.0 kg)
|
ELISA Reagents include Coating Antibody and Enzyme Conjugate and all plates.
ELISA Kits include Coating Antibody and Enzyme Conjugate, all Buffer sets, Positive/Negative controls and all plates.
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To know more about the products: Product Items
To learn how to perform the test: Instructions for DAS ELISA
To be familiar with other components: Buffer Sets; Controls
Information About the Virus
Name: Hibiscus Chlorotic Ringspot Virus
Acronym: HCRSV
Synonyms:
Group/Genus: Carmovirus
Vector: Transmitted by means not involving a vector.
Transmission: Transmitted by mechanical inoculation.
Main natural host plants: Hibiscus rosa-sinensis; Abelmoschus manihot.
Virus Infection: Symptoms by viral infection include mottling or chlorotic spots, rings, vein-banding or mosaic, very variable. Symptoms persist.
Diagnostically susceptible host species and symptoms: Chenopodium quinoa - chlorotic local lesions, not systemic; Cyamopsis tetragonoloba, Gossypium hirsutum, Vigna unguiculata ssp. cylindrica - necrotic local lesions, not systemic; Hibiscus cannabinus (kenaf) - ringspots, local lesions, then systemic mottle; Phaseolus vulgaris cv. Pinto - small local lesions, not systemic.
Geographical distribution: Spreads in Australia, El Salvador, Fiji, the Solomon Islands, Thailand, and the USA.
References:
1. Brunt, A.A., Barton, R.J., Phillips, S. and Lana, A.O. (1980). Ann. appl. Biol. 96: 37.
2. Waterworth, H.E. (1980). CMI/AAB Descr. Pl. Viruses No. 227, 4 pp.