Lettuce Big Vein Associated Virus (LBVaV) - DAS ELISA


Catalog #:  V274


Source Antibody:
  Rabbit polyclonal antibody for both capture and detection.
Test Format:    DAS ELISA


Reactivity:  Reaction of the ELISA is moderately strong.  Optical Density at 405nm is in a range of 0.650 - 1.750 depending on the virus titer in the samples tested.


Sensitivity:  Sensitivity of the ELISA is relatively high.  The virus can be consistently detected in infected plant tissues diluted at 1:270 - 810.


Specificity:  This test system has no cross reaction with healthy plant tissues tested. Background is low on the negative control wells.

MiLBVV-P


Products: 


Catalog Number

Description

Test Wells

Price $USD

Ship Weight
(Estimated)

V274-R1

ELISA Reagents, Alkaline Phosphatase

500

320

2 lb (1 kg)

V274-R2

ELISA Reagents, Alkaline Phosphatase

1000

560

3 lb (1.5 kg)

V274-R3

ELISA Reagents, Alkaline Phosphatase

5000

2300

12 lb (6 kg)

V274-R4

ELISA Reagents, Alkaline Phosphatase

100

160

1 lb (0.5 kg)

V274-K1

ELISA Kit, Alkaline Phosphatase

500

480

2 lb (1 kg)

V274-K2

ELISA Kit, Alkaline Phosphatase

1000

800

3 lb (1.5 kg)

V274-C1

Coating Antibody

500

170

2 lb (1 kg)

V274-C2

Coating Antibody

1000

320

2 lb (1 kg)

V274-C3

Coating Antibody

5000

1380

2 lb (1 kg)

V274-D1

Detecting conjugate, Alkaline Phosphatase

500

220

2 lb (1 kg)

V274-D2

Detecting conjugate, Alkaline Phosphatase

1000

380

2 lb (1 kg)

V274-D3

Detecting conjugate, Alkaline Phosphatase

5000

1600

2 lb (1 kg)

V274-P1

ELISA control, positive

18

28

1 lb (0.5 kg)

V274-N1

Negative control

18

28

1 lb (0.5 kg)

M004-1

Buffers for DAS/TAS, alkaline phosphatase

     500

130

2 lb (1.0 kg)

M004-2

Buffers for DAS/TAS, alkaline phosphatase

    1000 

220

3 lb (1.5 kg)

M004-3

Buffers for DAS/TAS, alkaline phosphatase

    5000 

900

6 lb (3.0 kg)

DELISA Reagents include Coating Antibody and Enzyme Conjugateand all plates.

 

D

ELISA Kits include Coating Antibody and Enzyme Conjugate, all Buffer sets, Positive/Negative controls and all plates.

 

It is easy and convenient: To Place An Order 
To know more about the products:
  Product Items
To learn how to perform the test:
  Instructions for DAS ELISA
To be familiar with other components: Buffer Sets; Controls
Information About the Virus
Name: Lettuce Big Vein Associated Virus
Acronym:  LBVaV
Synonyms:
Genus: Varicosavirus
Main Diseases: Symptoms vary seasonally. Symptoms vein yellowing then chlorotic or white vein banding. Infection of Lactuca sativa show chlorotic or white vein banding.
Transmission: Transmitted by a vector; a fungus; Olpidium brassicae; Chytridiales. Not transmitted by Myzus persicae, Hyperomyzus lactuca, Xiphinema spp., Longidorus spp. Virus transmitted by mechanical inoculation; transmitted by grafting; not transmitted by contact between plants; not transmitted by seed; not transmitted by pollen.
Serological Relationship: The virions of this virus are closely serological related to those of tobacco stunt virus, but as they differ in host range, they are considered to be separate species, rather than related strains (Kuwata and Kubo, 1986).
Geographical Distribution: Spreads in Australia, the former Czechoslovakia, Italy, Japan, the Netherlands, New Zealand, the UK, and the USA.
References:
1.
Allen, M.W. (1948). Phytopathology 38: 616.
2. Campbell, R.N. (1985). Can. J. Bot. 63: 2288.
3. Tomlinson, J.A. and Garrett, R.G. (1964). Ann. appl. Biol. 54: 45.
4. Umar M, Amer MA, Al-Saleh MA, Al-Shahwan IM, Shakeel MT, Zakri AM, Katis NI. Arch Virol. 2017 Jul;162(7):2067-2072.
5. Vetten, H.J., Lesemann, D.-E. and Dalchow, J. (1987). J. Phytopath. 120: 53.


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