Mirafiori Lettuce Big Vein Virus (MiLBVV) - DAS ELISA
Catalog #: V275
Source Antibody: Rabbit polyclonal antibody for both capture and detection.
Test Format: DAS ELISA
Reactivity: Reaction of the ELISA is strong. Optical Density at 405nm is in a range of 0.900 – 2.400 depending on the virus titer in the samples tested.
Sensitivity: Sensitivity of the ELISA is high. The virus can be consistently detected in infected plant tissues diluted at 1:810 - 2430.
Specificity: This test system has no cross reaction with healthy plant tissues tested. Background is low on the negative control wells.
Products:
Catalog Number |
Description |
Test Wells |
Price $USD |
Ship Weight |
V275-R1 |
ELISA Reagents, Alkaline Phosphatase |
500 |
320 |
2 lb (1 kg) |
V275-R2 |
ELISA Reagents, Alkaline Phosphatase |
1000 |
560 |
3 lb (1.5 kg) |
V275-R3 |
ELISA Reagents, Alkaline Phosphatase |
5000 |
2300 |
12 lb (6 kg) |
V275-R4 |
ELISA Reagents, Alkaline Phosphatase |
100 |
160 |
1 lb (0.5 kg) |
V275-K1 |
ELISA Kit, Alkaline Phosphatase |
500 |
480 |
2 lb (1 kg) |
V275-K2 |
ELISA Kit, Alkaline Phosphatase |
1000 |
800 |
3 lb (1.5 kg) |
V275-C1 |
Coating Antibody |
500 |
170 |
2 lb (1 kg) |
V275-C2 |
Coating Antibody |
1000 |
320 |
2 lb (1 kg) |
V275-C3 |
Coating Antibody |
5000 |
1380 |
2 lb (1 kg) |
V275-D1 |
Detecting conjugate, Alkaline Phosphatase |
500 |
220 |
2 lb (1 kg) |
V275-D2 |
Detecting conjugate, Alkaline Phosphatase |
1000 |
380 |
2 lb (1 kg) |
V275-D3 |
Detecting conjugate, Alkaline Phosphatase |
5000 |
1600 |
2 lb (1 kg) |
V275-P1 |
ELISA control, positive |
18 |
28 |
1 lb (0.5 kg) |
V275-N1 |
Negative control |
18 |
28 |
1 lb (0.5 kg) |
M004-1 |
Buffers for DAS/TAS, alkaline phosphatase |
500 |
130 |
2 lb (1.0 kg) |
M004-2 |
Buffers for DAS/TAS, alkaline phosphatase |
1000 |
220 |
3 lb (1.5 kg) |
M004-3 |
Buffers for DAS/TAS, alkaline phosphatase |
5000 |
900 |
6 lb (3.0 kg) |
ELISA Reagents include Coating Antibody and Enzyme Conjugate.
ELISA Kits include Coating Antibody and Enzyme Conjugate, all Buffer sets, Positive/Negative controls and all plates.
It is easy and convenient: To Place An Order
To know more about the products: Product Items
To learn how to perform the test: Instructions for DAS ELISA
To be familiar with other components: Buffer Sets; Controls
Information About the Virus
Name: Mirafiori Lettuce Big Vein Virus
Acronym: MiLBVV
Synonyms:
Genus:
Main Diseases: Symptoms vary seasonally. Symptoms vein yellowing then chlorotic or white vein banding. Infection of Lactuca sativa show chlorotic or white vein banding.
Transmission: Transmitted by a vector; a fungus; Olpidium brassicae; Chytridiales. Not transmitted by Myzus persicae, Hyperomyzus lactuca, Xiphinema spp., Longidorus spp. Virus transmitted by mechanical inoculation; transmitted by grafting; not transmitted by contact between plants; not transmitted by seed; not transmitted by pollen.
Serological Relationship: The virions of this virus are closely serological related to those of tobacco stunt virus, but as they differ in host range, they are considered to be separate species, rather than related strains (Kuwata and Kubo, 1986).
Geographical Distribution: Spreads in Australia, the former Czechoslovakia, Italy, Japan, the Netherlands, New Zealand, the UK, and the USA.
References:
1. Allen, M.W. (1948). Phytopathology 38: 616.
2. Campbell, R.N. (1985). Can. J. Bot. 63: 2288.
3. Tomlinson, J.A. and Garrett, R.G. (1964). Ann. appl. Biol. 54: 45.
4. Umar M, Amer MA, Al-Saleh MA, Al-Shahwan IM, Shakeel MT, Zakri AM, Katis NI. Arch Virol. 2017 Jul;162(7):2067-2072.
5. Vetten, H.J., Lesemann, D.-E. and Dalchow, J. (1987). J. Phytopath. 120: 53.