Potato Aucuba Mosaic Virus (PAMV) - DAS ELISA                          Home    Products    Testing Services   Supplies

Catalog #:  V126
Source Antibody:  Rabbit polyclonal antibody for both capture and detection.
Test Format:  DAS ELISA                                   

Reactivity:  This test system reacted with all virus isolates tested.  Reaction of the ELISA is moderate.  Optical Density at 405nm  is in arrange of 0.700 - 1.200 depending  on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is relatively high.  The virus can be consistently detected in infected plant tissues diluted at 1:270 - 1:810.

Specificity:  There is no obvious cross reaction with healthy plant  tissues.  Background is low on the negative control wells incubated with healthy plant sample or sample buffer.

Application:  The test can be used to detect PAMV in infected host plants.

Products: 

Catalog Number

Description

Test Wells Price $USD Ship Weight
(Estimated)

V126-R1

ELISA Reagents, Alkaline Phosphatase

500

275

2 lb (1 kg)

V126-R2

ELISA Reagents, Alkaline Phosphatase

1000

470

3 lb (1.5 kg)

V126-R3

ELISA Reagents, Alkaline Phosphatase

5000

1860

12 lb (6 kg)
V126-R4 ELISA Reagents, Alkaline Phosphatase

100

105

1 lb (0.5 kg)
V126-K1 ELISA Kit, Alkaline Phosphatase

500

435

2 lb (1 kg)
V126-K2 ELISA Kit, Alkaline Phosphatase

1000

690

3 lb (1.5 kg)

V126-C1

Coating Antibody

500

140

2 lb (1 kg)

V126-C2

Coating Antibody

1000

260

2 lb (1 kg)

V126-C3

Coating Antibody

5000

885

2 lb (1 kg)

V126-D1

Detecting conjugate, Alkaline Phosphatase

500

190

2 lb (1 kg)

V126-D2

Detecting conjugate, Alkaline Phosphatase

1000

315

2 lb (1 kg)

V126-D3

Detecting conjugate, Alkaline Phosphatase

5000

1080

2 lb (1 kg)
V126-P2 ELISA control, Positive

18

20

1 lb (0.5 kg)

V126-N1

Negative control

18

20

1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase      500
130
2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase     1000 
220
3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase     5000 
900
6 lb (3.0 kg)

DELISA Reagents include Coating Antibody and Enzyme Conjugate.

 

D

ELISA Kits include Coating Antibody and Enzyme Conjugate, all Buffer sets, Positive/Negative controls and all plates.

It is easy and convenient To Place An Order
To know more about the products:
  Product Items
To learn how to perform the test:
  Instructions for DAS ELISA
To be familiar with other components:  Instructions for DAS ELISA

Information About the Virus

Name:  Potato Aucuba Mosaic Virus
Acronym:  PAMV
Synonyms:  
Group/Genus:  Potexviruses

Vector:  
Transmission:  by a vector; an insect; Myzus persicae; Aphididae, and by mechanical transmission.
Main host plants:  Solanum tuberosum - chlorotic local lesions on older leaves, necrotic on younger, systemic (top) necrosis (Clinch, 1941). Stored tubers develop necrosis in the cortex and pith with irregular brown or sunken patches on the surface

Virus Infection:  cause chlorotic local lesions on older leaves, necrotic on younger, systemic (top) necrosis. Stored tubers develop necrosis in the cortex and pith with irregular brown or sunken patches on the surface.

References:
1. 
Kassanis, B. and Govier, D.A. (1972). CMI/AAB Descr. Pl. Viruses No. 98, 4 pp.
2.  Harrison, B.D. and Jones, R.A.C. (1970). Ann. appl. Biol. 65: 393.


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