Pelargonium Leaf Curl Virus (PLCV) - DAS ELISA Home    Products    Testing Services   Supplies   Contact Us

Catalog #:  V284
Source Antibody:
  Rabbit polyclonal antibody for both capture and detection.
Test Format:    DAS ELISA
Reactivity:  Reaction of the ELISA is strong.  Optical Density at 405nm is in a range of 0.800 - 2.300 depending on the virus titer in the samples tested.
Sensitivity:  Sensitivity of the ELISA is high.  The virus can be consistently detected in infected plant tissues diluted at 1:270 - 1:810.
Specificity:  This test system has no cross reaction with healthy plant tissues tested. Background is relatively low on the negative control wells.
Products: 


Catalog Number

Description

Test Wells

Price USD $

Ship Weight
(Estimated)

V284-R1

ELISA Reagents, Alkaline Phosphatase

500

320

2 lb (1 kg)

V284-R2

ELISA Reagents, Alkaline Phosphatase

1000

560

3 lb (1.5 kg)

V284-R3

ELISA Reagents, Alkaline Phosphatase

5000

2300

12 lb (6 kg)

V284-R4

ELISA Reagents, Alkaline Phosphatase

100

160

1 lb (0.5 kg)

V284-K1

ELISA Kit, Alkaline Phosphatase

500

480

2 lb (1 kg)

V284-K2

ELISA Kit, Alkaline Phosphatase

1000

800

3 lb (1.5 kg)

V284-C1

Coating Antibody

500

170

2 lb (1 kg)

V284-C2

Coating Antibody

1000

320

2 lb (1 kg)

V284-C3

Coating Antibody

5000

1380

2 lb (1 kg)

V284-D1

Detecting conjugate, Alkaline Phosphatase

500

220

2 lb (1 kg)

V284-D2

Detecting conjugate, Alkaline Phosphatase

1000

380

2 lb (1 kg)

V284-D3

Detecting conjugate, Alkaline Phosphatase

5000

1600

2 lb (1 kg)

V284-P2

ELISA control, Positive

18

28

1 lb (0.5 kg)

V284-N1

Negative control

18

28

1 lb (0.5 kg)

M004-1

Buffers for DAS/TAS, alkaline phosphatase

500

130

2 lb (1.0 kg)

M004-2

Buffers for DAS/TAS, alkaline phosphatase

1000 

220

3 lb (1.5 kg)

M004-3

Buffers for DAS/TAS, alkaline phosphatase

5000 

900

6 lb (3.0 kg)

DELISA Reagents include Coating Antibody and Enzyme Conjugate.

 

D

ELISA Kits include Coating Antibody and Enzyme Conjugate, all Buffer sets, Positive/Negative controls and all plates.

It is easy and convenient: To Place An Order 
To know more about the products:
  Product Items
To learn how to perform the test:
  Instructions for DAS ELISA
To be familiar with other components: Buffer Sets; Controls
Information About the Virus
Name: Pelargonium Leaf Curl Virus
Acronym:  PLCV
Genus: Tombusvirus
The virus: PLCV was first reported in Pelargonium zonale from Germany by Pape in 1927. Virions is isometric, not enveloped with 28 nm in diameter. Viral genome consists of single-stranded RNA with total genome size 4.7 kb.
Main host plants and Symptoms: The symptoms on Pelargonium zonale include pale chlorotic spots which later become stellate and necrotic, leaf crinkling and plant degeneration.
Transmission: PLCV virus is transmitted by mechanical inoculation, and also transmitted by grafting. But is not transmitted by seed.
Geographical Distribution: PLCV spreads in many countries of the Eurasian region, the Mediterranean region, and the North American region.
References:
1. Hollings, M. and Stone, O.M. (1965). Ann. appl. Biol. 56: 87.
2. Martelli, G.P., Gallitelli, D. and Russo, M. (1988). In The Plant Viruses; 3, Polyhedral Virions with Monopartite Genomes, pp. 13-72. Plenum Press, New York.
3.  Wetter, C. and Luisoni, E. (1969). Phytopath. Z. 65: 231.

 

It is easy and convenient: To Place An Order 
To know more about the products:
  Product Items
To learn how to perform the test:
  Instructions for DAS ELISA
To be familiar with other components: Buffer Sets; Controls

 


Home    Products    Testing Services   Supplies   Contact Us