Phakopsora (Phak) - TAS ELISA                            Home    Products    Testing Services   Supplies

Catalog #:  F240
Source Antibody:  A rabbit polyclonal antibody for capture and a monoclonal antibody for detection.
Test Format: TAS ELISA         
                 
Reactivity:   This ELISA reacts with P. pachyrhizi and P. meibomiae fungal species. The assay detected the isolates of P. pachyrhizi and P. meibomiae ininfected host plants such as soybean and lima bean.
Reaction of this test is very strong.  Optical Density at 405nm  is in a range of 2.000 - >3.000 depending on the fungal titers in the samples tested.

Sensitivity: This ELISA is very sensitive, and can detect ASR/SR fungi in pre‐symptomatic plant leaves.   The ASR/SR pathogen can be detected at dilution of 1:21870 for the plant leaf samples prepared at 14 days after infection, and at dilution of 1:270 for the plant leaf samples prepared at 5 days after infection.

Specificity:  This test system is genius‐specific for Phakopsora, and reacts with both species of P. pachyrhizi (ASR) and P.meibomiae (SR). The antibodies used in this ELISA have high specificity to both r369 and r107 proteins.
This assaydoes not react with the other related rust fungi or any of the soybeanpathogens tested, including  Uromyces appendisulatus, Puccinia punctiformis, Puccinia graminis, Puccinia graminis tritici, Rose rust, Fusarium graminearum, Downy mildew, Pseudomonas syringae , Pseudomonas syringae pv. phaseolicola,  Xanthomonas campestris pv. glycine, Ustilago tritici, Erysiphae polygono DC, Septoria lycopersici and Cercospora sojina.
There is no cross reaction with healthy plant tissues.  Background is very low on all of the negative control wells.

Application:  The test can be used to detect the ASR/SR fungi in pre-symptomatic plant leaves, and to detect the pathogen in many host plant tissues infected with Phytophthora spp., such as leaves, stems, roots, seeds, and potato tubers, as well as in pure fungal cultures.

Immunodiagnostic assays targeted to urediniospore wall proteins of Asian soybean rust - USDA ARS Foreign Disease Weed Science Research Unit, Fort Detrick, MD

Products: 

ELISA Reagents include Coating Antibody and Enzyme Conjugate.

ELISA Kits include Coating Antibody and Enzyme Conjugate, all Buffer sets, Positive/Negative controls and all plates

It is easy and convenient To Place An Order
To know more about the products:  Product Items
To learn how to perform the test:   Instructions for TAS ELISA 
To be familiar with other components: Buffer Sets; Controls

Information About the Fungus

Name:  Phakopsora
Acronym:  Phak
Synonyms: 
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