Rice Hoja Blanca Virus (RHBV) - DAS ELISA Home Products Testing Services Supplies Contact Us
Catalog #: V180
Source Antibody: Rabbit polyclonal antibody for both capture and
detection.
Test Format: DAS ELISA
Reactivity: The test reacted with all RHBV isolates tested. Reaction of the ELISA is moderate strong. Optical Density at 405nm is in arrange of 0.600 - 2.400 depending on the virus titer in the samples tested.
Sensitivity: Sensitivity of the ELISA is moderate high. The virus can be consistently detected in infected plant tissues diluted at 1:810.
Specificity: There is no cross reaction with healthy plant tissues such as rice and other host plants. Background is relative low on all of the negative control wells.
Application: The test can be used to detect RHBV in infected host plants.
Products:
|
Catalog Number |
Description |
Test Wells | Price $USD |
Ship
Weight (Estimated) |
|
V180-R1 |
ELISA Reagents, Alkaline Phosphatase |
500 |
275 |
2 lb (1 kg) |
|
V180-R2 |
ELISA Reagents, Alkaline Phosphatase |
1000 |
470 |
3 lb (1.5 kg) |
|
V180-R3 |
ELISA Reagents, Alkaline Phosphatase |
5000 |
1860 |
12 lb (6 kg) |
| V180-R4 | ELISA Reagents, Alkaline Phosphatase |
100 |
105 |
1 lb (0.5 kg) |
| V180-K1 | ELISA Kit, Alkaline Phosphatase |
500 |
435 |
2 lb (1 kg) |
| V180-K2 | ELISA Kit, Alkaline Phosphatase |
1000 |
690 |
3 lb (1.5 kg) |
|
V180-C1 |
Coating Antibody |
500 |
140 |
2 lb (1 kg) |
|
V180-C2 |
Coating Antibody |
1000 |
260 |
2 lb (1 kg) |
|
V180-C3 |
Coating Antibody |
5000 |
885 |
2 lb (1 kg) |
|
V180-D1 |
Detecting conjugate, Alkaline Phosphatase |
500 |
190 |
2 lb (1 kg) |
|
V180-D2 |
Detecting conjugate, Alkaline Phosphatase |
1000 |
315 |
2 lb (1 kg) |
|
V180-D3 |
Detecting conjugate, Alkaline Phosphatase |
5000 |
1080 |
2 lb (1 kg) |
|
V180-P2 |
ELISA Control, Positive |
18 |
20 |
1 lb (0.5 kg) |
|
V180-N1 |
Negative control |
18 |
20 |
1 lb (0.5 kg) |
| M004-1 | Buffers for DAS/TAS, alkaline phosphatase | 500 | 130 |
2 lb (1.0 kg) |
| M004-2 | Buffers for DAS/TAS, alkaline phosphatase | 1000 | 220 |
3 lb (1.5 kg) |
| M004-3 | Buffers for DAS/TAS, alkaline phosphatase | 5000 | 900 |
6 lb (3.0 kg) |
ELISA Reagents include Coating Antibody and Enzyme Conjugate and all plates.
ELISA Kits include Coating Antibody and Enzyme Conjugate, all Buffer sets, Positive/Negative controls and all plates.
It is easy and convenient To Place
An Order
To know more about the products: Product
Items
To learn how to perform the test:
Instructions for DAS ELISA
To be familiar with other components: Buffer
Sets; Controls
Information About the Virus
Name: Rice Hoja Blanca Virus
Acronym: RHBV
Synonyms: echinochloa hoja blanca virus (Falk et al.,
1987)
Group/Genus: Tenuivirus
Vector: Transmitted by a vector; an insect; Sogatodes orizicola;
Delphacidae. Transmitted in a persistent manner. Virus retained when the vector
moults; multiplies in the vector; transmitted congenitally to the progeny of the
vector.
Transmission: Virus not transmitted by mechanical inoculation; not
transmitted by contact between plants; not transmitted by seed.
Main host plants: Oryza sativa, wild Oryza spp.
Virus Infection: Cause symptoms of systemic necrosis, systemic
chlorotic striping in early infections.
Symptoms persist.
Diagnostically susceptible host species and symptoms: Avena sativa,
Digitaria spp., Hordeum vulgare, Leptochloa spp., Oryza sativa
cv. Bluebonnet-50, Secale cereale, Triticum aestivum - chlorotic stripes.
Geographical distribution: Spreads in the South and Central American region; Colombia, Costa Rica, Cuba, the Dominican Republic, Ecuador, El Salvador, Honduras, Nicaragua, Panama, Peru, and Venezuela (and elsewhere in the Caribbean). Found, but with no evidence of spread, in the U.S.A.
References:
1. Morales, F.J. and Niessen, A.I. (1985). AAB Descr. Pl. Viruses
No. 299, 4 pp.
2. Ramirez, B.C., Lozano, I., Constantino, L.-M., Haenni, A.-L. and
Calvert, L.A. (1993). J. gen. Virol. 74: 2463.