Wheat Soil-Borne Mosaic Virus (SBWMV) - TAS ELISA Home Products Testing Services Supplies
Catalog #: V204
Source Antibody: A rabbit polyclonal antibody for capture and a monoclonal antibody for detection.
Test Format: TAS ELISA
Reactivity: This test system reacted with all SBWMV isolates tested. Reaction of the ELISA is strong. Optical Density at 405nm is in a range of 0.900 - 2.900 depending on the virus titer in the samples tested.
Sensitivity: Sensitivity of the ELISA is high. The virus can be consistently detected in infected plant leaf tissues diluted at 1:810-1:2430.
Specificity: There is no cross reaction with healthy plant tissues such as wheat, barley, corn or other host plants. Background is very low on all of the negative control wells
Application: The test can be used to detect SBWMV in infected host plants.
Products:
|
Catalog Number |
Description |
Test Wells | Price $USD | Ship Weight (Estimated) |
|
V204-R1 |
ELISA Reagents, Alkaline Phosphatase |
500 |
275 |
2 lb (1 kg) |
|
V204-R2 |
ELISA Reagents, Alkaline Phosphatase |
1000 |
470 |
3 lb (1.5 kg) |
|
V204-R3 |
ELISA Reagents, Alkaline Phosphatase |
5000 |
1860 |
12 lb (6 kg) |
| V204-R4 | ELISA Reagents, Alkaline Phosphatase |
100 |
105 |
1 lb (0.5 kg) |
| V204-K1 | ELISA Kit, Alkaline Phosphatase |
500 |
435 |
2 lb (1 kg) |
| V204-K2 | ELISA Kit, Alkaline Phosphatase |
1000 |
690 |
3 lb (1.5 kg) |
|
V204-C1 |
Coating Antibody |
500 |
140 |
2 lb (1 kg) |
|
V204-C2 |
Coating Antibody |
1000 |
260 |
2 lb (1 kg) |
|
V204-C3 |
Coating Antibody |
5000 |
885 |
2 lb (1 kg) |
|
V204-D1 |
Detecting conjugate, Alkaline Phosphatase |
500 |
190 |
2 lb (1 kg) |
|
V204-D2 |
Detecting conjugate, Alkaline Phosphatase |
1000 |
315 |
2 lb (1 kg) |
|
V204-D3 |
Detecting conjugate, Alkaline Phosphatase |
5000 |
1080 |
2 lb (1 kg) |
|
V204-P1 |
Positive control |
18 |
20 |
1 lb (0.5 kg) |
|
V204-N1 |
Negative control |
18 |
20 |
1 lb (0.5 kg) |
| M004-1 | Buffers for DAS/TAS, alkaline phosphatase | 500 | 110 | 2 lb (1.0 kg) |
| M004-2 | Buffers for DAS/TAS, alkaline phosphatase | 1000 | 145 | 3 lb (1.5 kg) |
| M004-3 | Buffers for DAS/TAS, alkaline phosphatase | 5000 | 440 | 6 lb (3.0 kg) |
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To know more about the products: Product Items
To learn how to perform the test: Instructions for DAS ELIAS
To be familiar with other components: Buffer Sets; Controls
Information About the Virus
Name: Wheat Soil-Borne Mosaic Virus
Acronym: SBWMV
Synonyms: wheat viruses 1 and 2, soil-borne wheat green mosaic virus, wheat mosaic virus.
Group/Genus: Furoviruses
Vector: Transmitted by a vector; a fungus; Polymyxa graminis; Estes and Brakke (1966); Plasmodiophorales (Brakke, 1971).
Transmission: Virus transmitted by mechanical inoculation; not transmitted by seed
Main host plants: Triticum aestivum (winter wheat) and Hordeum vulgare.
Virus Infection Symptom: light green to yellow leaf mosaic, roots stunted, severe rosetting and tillering of susceptible cultivars (Brakke, 1971).
Diagnostically susceptible host species and symptoms: Triticum aestivum and other Triticum spp. - mosaic and rosetting.
Geographical distribution: Spreads in China, Italy, Japan, and the USA (Cai et al. (1983); Canova (1966); Putnam et al. (1994); Saito et al. (1964)).
Reference:
1. Brakke, M.K. (1971). CMI/AAB Descr. Pl. Viruses No. 77. 4 pp.
2. Putnam, M.L., Carroll, T.W. and French, R. (1994). Plant Dis. 78: 102.
3. Shirako, Y. and Wilson, T.M.A. (1992). Virology 195: 16.