Sweet Potato Mild Mottle Virus (SPMMV) - DAS ELISA Home Products Testing Services Supplies Contact Us
Catalog #: V286
Source Antibody: Rabbit polyclonal antibody for both capture and detection.
Test Format: DAS ELISA
Reactivity: Reaction of the ELISA is moderate strong. Optical Density at 405nm is in a range of 0.300 – 1.300 depending on the virus titer in the samples tested.
Sensitivity: Sensitivity of the ELISA is relatively high. The virus can be consistently detected in infected plant tissues diluted at 1:90 - 1:810.
Specificity: This test system has no cross reaction with healthy plant tissues tested. Background is low on the negative control wells.
Products:
Catalog Number |
Description |
Test Wells |
Price USD $ |
Ship Weight |
V286-R1 |
ELISA Reagents, Alkaline Phosphatase |
500 |
320 |
2 lb (1 kg) |
V286-R2 |
ELISA Reagents, Alkaline Phosphatase |
1000 |
560 |
3 lb (1.5 kg) |
V286-R3 |
ELISA Reagents, Alkaline Phosphatase |
5000 |
2300 |
12 lb (6 kg) |
V286-R4 |
ELISA Reagents, Alkaline Phosphatase |
100 |
160 |
1 lb (0.5 kg) |
V286-K1 |
ELISA Kit, Alkaline Phosphatase |
500 |
480 |
2 lb (1 kg) |
V286-K2 |
ELISA Kit, Alkaline Phosphatase |
1000 |
800 |
3 lb (1.5 kg) |
V286-C1 |
Coating Antibody |
500 |
170 |
2 lb (1 kg) |
V286-C2 |
Coating Antibody |
1000 |
320 |
2 lb (1 kg) |
V286-C3 |
Coating Antibody |
5000 |
1380 |
2 lb (1 kg) |
V286-D1 |
Detecting conjugate, Alkaline Phosphatase |
500 |
220 |
2 lb (1 kg) |
V286-D2 |
Detecting conjugate, Alkaline Phosphatase |
1000 |
380 |
2 lb (1 kg) |
V286-D3 |
Detecting conjugate, Alkaline Phosphatase |
5000 |
1600 |
2 lb (1 kg) |
V286-P2 |
ELISA control, Positive |
18 |
28 |
1 lb (0.5 kg) |
V286-N1 |
Negative control |
18 |
28 |
1 lb (0.5 kg) |
M004-1 |
Buffers for DAS/TAS, alkaline phosphatase |
500 |
130 |
2 lb (1.0 kg) |
M004-2 |
Buffers for DAS/TAS, alkaline phosphatase |
1000 |
220 |
3 lb (1.5 kg) |
M004-3 |
Buffers for DAS/TAS, alkaline phosphatase |
5000 |
900 |
6 lb (3.0 kg) |
ELISA Reagents include Coating Antibody and Enzyme Conjugate.
ELISA Kits include Coating Antibody and Enzyme Conjugate, all Buffer sets, Positive/Negative controls and all plates.
Information About the Virus
Name: Sweet Potato Mild Mottle Virus
Acronym: SPMMV
Genus: Ipomovirus
The virus: SPMMV is first reported in sweet potato (Ipomoea batatas) from Kenya, Uganda and Tanzania by Sheffield in 1957. The virion is filamentous with length of 800-950 nm. Viral genome consists of single-stranded RNA containing 5 % nucleic acid and 95 % protein.
Main host plants and Symptoms: SPMMV infects sweet potato showing mild leaf mottling and stunting in intolerant cultivars, but symptomless infection in others. Some cultivars are immune.
Transmission: The virus is transmitted by a insect vector, whitefly (Bemisia tabaci, Aleyrodidae) in a persistent manner. Virus is also transmitted by mechanical inoculation and by grafting. It is not transmitted by contact between plants, and not transmitted by seed.
Geographical Distribution: SPMMV is spread in Burundi, Kenya, Tanzania, and Uganda, and possible some other regions in the worlds.
References:
1. Hollings, M., Stone, O.M. and Bock, K.R. (1971). Rep. Glasshouse Crops Res. Inst. 1970, p. 155.
2. Sheffield, F.M.L. (1957). Phytopathology 47: 582.
3. Wambugu, F. (1990). Ph.D. Thesis, pp. 271. University of Bath, U.K.
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To know more about the products: Product Items
To learn how to perform the test: Instructions for DAS ELISA
To be familiar with other components: Buffer Sets; Controls