Soybean Dwarf Virus (SbDV) - TAS ELISA Home Products Testing Services Supplies Contact Us
Catalog #: V155
Source Antibody: A rabbit polyclonal antibody for capture and and a monoclonal antibody as detection.
Test Format: TAS ELISA
Reactivity: The test reacted with all SbDV isolates tested. Reaction of the ELISA is moderate strong. Optical Density at 405nm is in arrange of 0.800 - >2.600 depending on the virus titer in the samples tested.
Sensitivity: Sensitivity of the ELISA is moderate high. The virus can be consistently detected in infected plant tissues diluted at 1:810.
Specificity: There is no cross reaction with healthy plant tissues such as soybean or other host plants. Background is very on all of the negative control wells.
Application: The test can be used to detect SbDV in infected host plant tissues.
Products:
|
Catalog Number |
Description |
Test Wells | Price $USD | Ship Weight (Estimated) |
|
V155-R1 |
ELISA Reagents, Alkaline Phosphatase |
500 |
275 |
2 lb (1 kg) |
|
V155-R2 |
ELISA Reagents, Alkaline Phosphatase |
1000 |
470 |
3 lb (1.5 kg) |
|
V155-R3 |
ELISA Reagents, Alkaline Phosphatase |
5000 |
1860 |
12 lb (6 kg) |
| V155-R4 | ELISA Reagents, Alkaline Phosphatase |
100 |
105 |
1 lb (0.5 kg) |
| V155-K1 | ELISA Kit, Alkaline Phosphatase |
500 |
435 |
2 lb (1 kg) |
| V155-K2 | ELISA Kit, Alkaline Phosphatase |
1000 |
690 |
3 lb (1.5 kg) |
|
V155-C1 |
Coating Antibody |
500 |
140 |
2 lb (1 kg) |
|
V155-C2 |
Coating Antibody |
1000 |
260 |
2 lb (1 kg) |
|
V155-C3 |
Coating Antibody |
5000 |
885 |
2 lb (1 kg) |
|
V155-D1 |
Detecting conjugate, Alkaline Phosphatase |
500 |
190 |
2 lb (1 kg) |
|
V155-D2 |
Detecting conjugate, Alkaline Phosphatase |
1000 |
315 |
2 lb (1 kg) |
|
V155-D3 |
Detecting conjugate, Alkaline Phosphatase |
5000 |
1080 |
2 lb (1 kg) |
|
V155-P2 |
ELISA control, positive |
18 |
20 |
1 lb (0.5 kg) |
|
V155-N1 |
Negative control |
18 |
20 |
1 lb (0.5 kg) |
| M004-1 | Buffers for DAS/TAS, alkaline phosphatase | 500 | 110 |
2 lb (1.0 kg)
|
| M004-2 | Buffers for DAS/TAS, alkaline phosphatase | 1000 | 145 |
3 lb (1.5 kg)
|
| M004-3 | Buffers for DAS/TAS, alkaline phosphatase | 5000 | 440 |
6 lb (3.0 kg)
|
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To know more about the products: Product Items
To learn how to perform the test: Instruction for TAS ELISA Reagents
To be familiar with other components: Buffer Sets; Controls
Information About the Virus
Name: Soybean Dwarf Virus
Acronym: SbDV
Synonyms: subterranean clover red leaf luteovirus
Group/Genus: Luteovirus
Vector: Transmitted by a vector; an insect; Acyrthosiphon (Aulacorthum) solani (some isolates also by Acyrthosiphon pisum); Aphididae. Transmitted in a persistent manner. Virus retained when the vector moults; does not multiply in the vector; not transmitted congenitally to the progeny of the vector; does not require a helper virus for vector transmission.
Transmission: Virus not transmitted by mechanical inoculation; not transmitted by contact between plants; not transmitted by seed.
Main natural host plants and symptoms: Symptoms persist.
- Glycine max, Trifolium pratense - puckering, rugosity and yellowing, stunting.
- Pisum sativum - mild yellowing of older leaves.
- Phaseolus vulgaris - yellowing of leaves and stunting.
- Gomphrena globosa, Erodium moschatum, Trifolium subterraneum, Vicia sativa, Calandrinia caulescens - leaf reddening.
- Beta vulgaris, Lupinus albus, Lupinus angustifolius, Medicago polymorpha - leaf yellowing.
- Trifolium repens, T. pratense - no symptoms.
- Vicia faba, Vicia articulata - leaf yellowing, rolling.
Diagnostically susceptible host species and symptoms: Astragalus sinicus - systemic leaf yellowing or reddening. Glycine max - systemic puckering, rugosity and yellowing of leaves, stunting. Trifolium incarnatum, T. subterraneum - systemic leaf reddening. Vicia faba - systemic chlorosis and yellowing.
Geographical distribution: Spreads in Australia, Japan, New Zealand, and the USA (in California).
References:
1. Johnstone, G.R., Duffus, J.E., Munro, D. and Ashby, J.W. (1982). Aust. J. agric. Res. 33: 697.
2. Tamada, T. and Kojima, M. (1977). CMI/AAB Descr. Pl. Viruses No. 179, 4 pp.