Tobacco Etch Virus (TEV) - DAS ELISA Home Products Testing Services Supplies Contact Us
Catalog #: V140
Source Antibody: Rabbit polyclonal antibody for both capture and detection.
Test Format: DAS ELISA
Reactivity: The test reacted with all TEV isolates tested. Reaction of the ELISA is strong. Optical Density at 405nm is in arrange of 1.200 - >2.900 depending on the virus titer in the samples tested.
Sensitivity: Sensitivity of the ELISA is high. The virus can be consistently detected in infected plant tissues diluted at 1:810 - 1:2430.
Specificity: There is no cross reaction with healthy plant tissues such as tobacco, tomato or other host plants. Background is very low on all of the negative control wells.
Application: The test can be used to detect TEV in infected host plants.
Products:
|
Catalog Number |
Description |
Test Wells | Price $USD | Ship Weight (Estimated) |
|
V140-R1 |
ELISA Reagents, Alkaline Phosphatase |
500 |
275 |
2 lb (1 kg) |
|
V140-R2 |
ELISA Reagents, Alkaline Phosphatase |
1000 |
470 |
3 lb (1.5 kg) |
|
V140-R3 |
ELISA Reagents, Alkaline Phosphatase |
5000 |
1860 |
12 lb (6 kg) |
| V140-R4 | ELISA Reagents, Alkaline Phosphatase |
100 |
105 |
1 lb (0.5 kg) |
| V140-K1 | ELISA Kit, Alkaline Phosphatase |
500 |
435 |
2 lb (1 kg) |
| V140-K2 | ELISA Kit, Alkaline Phosphatase |
1000 |
690 |
3 lb (1.5 kg) |
|
V140-C1 |
Coating Antibody |
500 |
140 |
2 lb (1 kg) |
|
V140-C2 |
Coating Antibody |
1000 |
260 |
2 lb (1 kg) |
|
V140-C3 |
Coating Antibody |
5000 |
885 |
2 lb (1 kg) |
|
V140-D1 |
Detecting conjugate, Alkaline Phosphatase |
500 |
190 |
2 lb (1 kg) |
|
V140-D2 |
Detecting conjugate, Alkaline Phosphatase |
1000 |
315 |
2 lb (1 kg) |
|
V140-D3 |
Detecting conjugate, Alkaline Phosphatase |
5000 |
1080 |
2 lb (1 kg) |
|
V140-P1 |
Positive control |
18 |
20 |
1 lb (0.5 kg) |
|
V140-N1 |
Negative control |
18 |
20 |
1 lb (0.5 kg) |
| M004-1 | Buffers for DAS/TAS, alkaline phosphatase | 500 | 130 | 2 lb (1.0 kg) |
| M004-2 | Buffers for DAS/TAS, alkaline phosphatase | 1000 | 200 | 3 lb (1.5 kg) |
| M004-3 | Buffers for DAS/TAS, alkaline phosphatase | 5000 | 900 | 6 lb (3.0 kg) |
ELISA Reagents include Coating Antibody and Enzyme Conjugate and all plates.
ELISA Kits include Coating Antibody and Enzyme Conjugate, all Buffer sets, Positive/Negative controls and all plates.
It is easy and convenient To Place An Order
To know more about the products: Product Items
To learn how to perform the test: Instructions for DAS ELISA
To be familiar with other components: Buffer Sets; Controls
Information About the Virus
Name: Tobacco Etch Virus
Acronym: TEV
Synonyms: datura Z virus, tobacco severe etch virus, tomato etch virus
Group/Genus: Potyvirus
Vector: Transmitted by a vector; an insect; more than 10 spp., especially Myzus persicae; Aphididae. Transmitted in a non-persistent manner. Virus does not require a helper virus for vector transmission.
Transmission: Virus transmitted by mechanical inoculation; not transmitted by seed.
Main host plants: Many natural host plants including Cassia obtusifolia, Capsicum annuum, Datura stramonium, Lycopersicon esculentum, Nicotiana tabacum, and Physalis spp.
Virus Infection: Symptoms persist and may be strain-dependant. Symptoms varies, including necrotic leaf etching, leaf mottling and distortion, mosaic, vein banding, malformation and necrosis, stunting and reduced seed yield.
Diagnostically susceptible host species and symptoms: Chenopodium album, C. amaranticolor and C. quinoa - local chlorotic or necrotic lesions, not systemic; Datura stramonium - systemic mosaic, ringspots, rugosity and leaf malformation
Geographical distribution: Spreads in the Eastern Asian region and the South and Central American region; Canada, China, Mexico, Puerto Rico, Venezuela, and the USA.
References:
1. Dougherty, W.G. and Hiebert, E. (1980). Virology 101: 466.
2. Purcifull, D.E. and Hiebert, E. (1982). CMI/AAB Descr. Pl. Viruses No. 258, 7 pp.