Tobacco Necrotic Dwarf Virus (TNDV) - DAS ELISA Home Products Testing Services Supplies Contact Us
Catalog #: V163
Source Antibody: Rabbit polyclonal antibody for both capture and
detection.
Test Format: DAS ELISA
Reactivity: The test reacted with all TNDV isolates tested. Reaction of the ELISA is moderate strong. Optical Density at 405nm is in arrange of 0.800 - >2.850 depending on the virus titer in the samples tested.
Sensitivity: Sensitivity of the ELISA is high. The virus can be consistently detected in infected plant tissues diluted at 1:810 - 1:2430.
Specificity: There is no cross reaction with healthy plant tissues such as tobacco or other host plants. Background is low on all of the negative control wells.
Application: The test can be used to detect TNDV in infected host plants.
Products:
|
Catalog Number |
Description |
Test Wells | Price $USD |
Ship
Weight (Estimated) |
|
V163-R1 |
ELISA Reagents, Alkaline Phosphatase |
500 |
275 |
2 lb (1 kg) |
|
V163-R2 |
ELISA Reagents, Alkaline Phosphatase |
1000 |
470 |
3 lb (1.5 kg) |
|
V163-R3 |
ELISA Reagents, Alkaline Phosphatase |
5000 |
1860 |
12 lb (6 kg) |
| V163-R4 | ELISA Reagents, Alkaline Phosphatase |
100 |
105 |
1 lb (0.5 kg) |
| V163-K1 | ELISA Kit, Alkaline Phosphatase |
500 |
435 |
2 lb (1 kg) |
| V163-K2 | ELISA Kit, Alkaline Phosphatase |
1000 |
690 |
3 lb (1.5 kg) |
|
V163-C1 |
Coating Antibody |
500 |
140 |
2 lb (1 kg) |
|
V163-C2 |
Coating Antibody |
1000 |
260 |
2 lb (1 kg) |
|
V163-C3 |
Coating Antibody |
5000 |
885 |
2 lb (1 kg) |
|
V163-D1 |
Detecting conjugate, Alkaline Phosphatase |
500 |
190 |
2 lb (1 kg) |
|
V163-D2 |
Detecting conjugate, Alkaline Phosphatase |
1000 |
315 |
2 lb (1 kg) |
|
V163-D3 |
Detecting conjugate, Alkaline Phosphatase |
5000 |
1080 |
2 lb (1 kg) |
|
V163-P2 |
ELISA control, positive |
18 |
20 |
1 lb (0.5 kg) |
|
V163-N1 |
Negative control |
18 |
20 |
1 lb (0.5 kg) |
| M004-1 | Buffers for DAS/TAS, alkaline phosphatase | 500 | 130 |
2 lb (1.0 kg) |
| M004-2 | Buffers for DAS/TAS, alkaline phosphatase | 1000 | 220 |
3 lb (1.5 kg) |
| M004-3 | Buffers for DAS/TAS, alkaline phosphatase | 5000 | 900 |
6 lb (3.0 kg) |
ELISA Reagents include Coating Antibody and Enzyme Conjugate and all plates.
ELISA Kits include Coating Antibody and Enzyme Conjugate, all Buffer sets, Positive/Negative controls and all plates.
It is easy and convenient To Place
An Order
To know more about the products: Product
Items
To learn how to perform the test: Instructions
for DAS ELISA
To be familiar with other components: Buffer
Sets; Controls
Information About the Virus
Name: Tobacco Necrotic Dwarf Virus
Acronym: TNDV
Synonyms:
Group/Genus: Luteovirus
Vector: Transmitted by a vector; an insect; Myzus persicae;
Aphididae. Transmitted in a persistent manner. Virus retained when the vector
moults; does not multiply in the vector; not transmitted congenitally to the
progeny of the vector.
Transmission: Virus is transmitted by grafting; not transmitted by
contact between plants; not transmitted by seed; not transmitted by pollen.
Main natural host plants: Spinacia oleracea, Nicotiana tabacum,
Capsella bursa-pastoris.
Virus Infection: Symptoms include clear vein yellowing, interveinal
leaf chlorosis, veinal necrosis, severe stunting, interveinal reddening,
stunting. Symptoms persist.
Diagnostically susceptible host species and symptoms: Capsella bursa-pastoris,
Datura stramonium, Nicotiana sylvestris, N. tabacum - systemic interveinal
chlorosis, veinal necrosis, stunting; Physalis floridana - systemic
interveinal chlorosis, leaf rolling; Montia perfoliata - mild systemic
interveinal chlorosis.
Geographical distribution: Spreads in Japan.
References:
1. Bock, K.R. and Kuhn, C.W. (1975). CMI/AAB Descr. Pl. Viruses
No. 141, 4 pp.
2. Morales, F.J., Castano, M., Velasco, A.C., Arroyave, J. and
Zettler, F.W. (1991). Plant Dis. 75: 1090.