Watermelon Chlorotic Stunt Virus (WmCSV) - DAS ELISA                 Home    Products    Testing Services   Supplies   Contact Us

Catalog #:  V269
Source Antibody:  A rabbit polyclonal antibody for both capture and detection.
Test Format:  DAS ELISA                                   

Reactivity:  The test reacted with all WmCSV isolates tested. Reaction of the ELISA is moderate strong.  Optical Density at 405nm  is in arrange of 0.400 - >1.700 depending on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is moderate high.  The virus can be consistently detected in infected plant tissues diluted  at 1:30 -1:90.

Specificity:  There is no cross reaction with healthy plant  tissues such as apple or other host plants. Background is low on all of  the negative control wells.

Application:  The test can be used to detect WmCSV in infected herbaceous host plant tissues.

Products: 

Catalog Number

Description

Test Wells Price $USD Ship Weight
(Estimated)

V269-R1

ELISA Reagents, Alkaline Phosphatase

500

320

2 lb (1 kg)

V269-R2

ELISA Reagents, Alkaline Phosphatase

1000

560

3 lb (1.5 kg)

V269-R3

ELISA Reagents, Alkaline Phosphatase

5000

2300

12 lb (6 kg)
V269-R4 ELISA Reagents, Alkaline Phosphatase

100

160

1 lb (0.5 kg)
V269-K1 ELISA Kit, Alkaline Phosphatase

500

480

2 lb (1 kg)
V269-K2 ELISA Kit, Alkaline Phosphatase

1000

800

3 lb (1.5 kg)

V269-C1

Coating Antibody

500

170

2 lb (1 kg)

V269-C2

Coating Antibody

1000

320

2 lb (1 kg)

V269-C3

Coating Antibody

5000

1380

2 lb (1 kg)

V269-D1

Detecting conjugate, Alkaline Phosphatase

500

220

2 lb (1 kg)

V269-D2

Detecting conjugate, Alkaline Phosphatase

1000

380

2 lb (1 kg)

V269-D3

Detecting conjugate, Alkaline Phosphatase

5000

1600

2 lb (1 kg)

V269-P2

ELISA control, positive

18

28

1 lb (0.5 kg)

V269-N1

Negative control

18

28

1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase 500
130
2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase
1000 
220
3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase
5000 
900
6 lb (3.0 kg)

DELISA Reagents include Coating Antibody and Enzyme Conjugate.

 

D

ELISA Kits include Coating Antibody and Enzyme Conjugate, all Buffer sets, Positive/Negative controls and all plates.

It is easy and convenient To Place An Order
To know more about the products:
  Product Items
To learn how to perform the test:
Instructions for DAS ELISA
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name:  Watermelon Chlorotic Stunt Virus
Acronym:  WmCSV
Synonyms:  Citrullus vulgaris, C. colocynthis - crinkling and chlorotic mottling of young leaves; severe stunting.
Group/Genus:  Bigeminvirus

Vector: Transmitted by a vector; an insect; probably Bemisia tabaci, but this has still to be confirmed; Aleyrodidae.
Transmission: irus not transmitted by mechanical inoculation; transmitted by grafting.
Main natural host plants:

Virus Infection Symptoms:
Diagnostically susceptible host species and symptoms:

  • Citrullus vulgaris - systemic chlorotic mottling, crinkling and malformation of tip leaves..

Geographical distribution: Spreads in Saudi Arabia (possibly), Yemen (the north and south).

References:

  • Alhubaishi, A.A., Walkey, D.G.A., Webb, M.J.W., Bolland, C.J. and Cook, A.A. (1987). FAO Pl. Prot. Bull. 35: 135.
  • Jones, P., Satar, M.H.A. and Alkaff, N. (1987). Rep. Rothamsted Exp. Stn. 1987, p. 76.
  • Jones, P., Sattar, M.H.A. and Alkaff, N. (1988). Asp. Appl. Biol. 17: 203.
  • Walkey, D.G.A., Alhubaishi, A.A. and Webb, M.J.W. (1989). Trop. Pest Manag. 36: 195.

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