Pelargonium Flower Break Virus (PFBV) - DAS ELISA                          Home    Products    Testing Services   Supplies

Catalog #:  V037
Source Antibody:  Rabbit polyclonal antibody for both capture and detection.
Test Format:  DAS ELISA                                   

Reactivity:  This test system reacted with all of the isolates of PFBV tested.  Reaction of the ELISA is relatively strong.  Optical Density at 405nm is in arrange of 0.900 - 2.200 depending on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is relatively high.  The virus can be consistently detected in infected plant tissues diluted at 1:810.

Specificity:  There is no obvious cross reaction found with healthy geranium tissues.  Background is relatively low on the negative control wells.

Application:  The test can be used to detect PFBV in infected geranium plants.

Products: 

Catalog Number

Description

Test Wells Price $USD Ship Weight
(Estimated)

V037-R1

ELISA Reagents, Alkaline Phosphatase

500

275

2 lb (1 kg)

V037-R2

ELISA Reagents, Alkaline Phosphatase

1000

470

3 lb (1.5 kg)

V037-R3

ELISA Reagents, Alkaline Phosphatase

5000

1860

12 lb (6 kg)
V037-R4 ELISA Reagents, Alkaline Phosphatase

100

105

1 lb (0.5 kg)
V037-K1 ELISA Kit, Alkaline Phosphatase

500

435

2 lb (1 kg)
V037-K2 ELISA Kit, Alkaline Phosphatase

1000

690

3 lb (1.5 kg)

V037-C1

Coating Antibody

500

140

2 lb (1 kg)

V037-C2

Coating Antibody

1000

260

2 lb (1 kg)

V037-C3

Coating Antibody

5000

885

2 lb (1 kg)

V037-D1

Detecting conjugate, Alkaline Phosphatase

500

190

2 lb (1 kg)

V037-D2

Detecting conjugate, Alkaline Phosphatase

1000

315

2 lb (1 kg)

V037-D3

Detecting conjugate, Alkaline Phosphatase

5000

1080

2 lb (1 kg)
V037-P1 Positive control

18

20

1 lb (0.5 kg)

V037-N1

Negative control

18

20

1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase      500
130
2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase     1000 
220
3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase     5000 
900
6 lb (3.0 kg)

DELISA Reagents include Coating Antibody and Enzyme Conjugate.

 

D

ELISA Kits include Coating Antibody and Enzyme Conjugate, all Buffer sets, Positive/Negative controls and all plates.

It is easy and convenient To Place An Order
To know more about the products:
  Product Items
To learn how to perform the test:
  Instructions for DAS ELIAS
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name:  Pelargonium Flower Break Virus
Acronym:  PFBV
Synonyms: 
Group/Genus:  Carmoviruses

Vector:  Unknown
Transmission:  Mechanical
Main host plants:  Geranium

Virus Particle:  
Virus Infection:
  Pelargonium flower break virus (PFBV) occurs widely in cultivated geraniums and are usually symptomless. Symptoms appearance in infected plants is greatly affected by various environmental factors. As the name of the virus suggests, main symptom of PFBV infection is color breaking on the flowers.  However, symptom of leaf chlorosis is also observed in some plants known to be infected with PFBV. 

Reference:
1.
CMI/AAB Descriptions of Plant Viruses, No. 130.


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