Papaya Ringspot Virus (PRSV) - DAS ELISA                                     Home    Products    Testing Services   Supplies

Catalog #:  V034
Source Antibody:  Rabbit polyclonal antibody for both capture and detection.
Test Format:  DAS ELISA                                   

Reactivity:  This test system reacted with all of the isolates of PRSV tested.  Reaction of the ELISA is very strong.  Optical Density at 405nm is in arrange of 1.200 - >3.000 depending on the virus titer in the samples tested.

Sensitivity:  Sensitivity of the ELISA is very high.  The virus can be consistently detected in infected plant tissues diluted at 1:2430.

Specificity:  There is no cross reaction found with healthy  plant tissues.  Background is very low on all of the negative control wells.

Application:  The test can be used to detect PRSV in infected host plants.

Products: 

Catalog Number

Description Test Wells Price $USD Ship Weight
(Estimated)

V034-R1

ELISA Reagents, Alkaline Phosphatase

500

275

2 lb (1 kg)

V034-R2

ELISA Reagents, Alkaline Phosphatase

1000

470

3 lb (1.5 kg)

V034-R3

ELISA Reagents, Alkaline Phosphatase

5000

1860

12 lb (6 kg)
V034-R4 ELISA Reagents, Alkaline Phosphatase

100

105

1 lb (0.5 kg)
V034-K1 ELISA Kit, Alkaline Phosphatase

500

435

2 lb (1 kg)
V034-K2 ELISA Kit, Alkaline Phosphatase

1000

690

3 lb (1.5 kg)

V034-C1

Coating Antibody

500

140

2 lb (1 kg)

V034-C2

Coating Antibody

1000

260

2 lb (1 kg)

V034-C3

Coating Antibody

5000

885

2 lb (1 kg)

V034-D1

Detecting conjugate, Alkaline Phosphatase

500

190

2 lb (1 kg)

V034-D2

Detecting conjugate, Alkaline Phosphatase

1000

315

2 lb (1 kg)

V034-D3

Detecting conjugate, Alkaline Phosphatase

5000

1080

2 lb (1 kg)

V034-P1

Positive control

18

20

1 lb (0.5 kg)

V034-N1

Negative control

18

20

1 lb (0.5 kg)
M004-1 Buffers for DAS/TAS, alkaline phosphatase 500
130
2 lb (1.0 kg)
M004-2 Buffers for DAS/TAS, alkaline phosphatase 1000 
220
3 lb (1.5 kg)
M004-3 Buffers for DAS/TAS, alkaline phosphatase 5000 
900
6 lb (3.0 kg)

DELISA Reagents include Coating Antibody and Enzyme Conjugate.

 

D

ELISA Kits include Coating Antibody and Enzyme Conjugate, all Buffer sets, Positive/Negative controls and all plates.

It is easy and convenient To Place An Order
To know more about the products:
  Product Items
To learn how to perform the test:
 Instructions for DAS ELISA
To be familiar with other components: Buffer Sets; Controls

Information About the Virus

Name:  Papaya Ringspot Virus
Acronym:  PRSV
Synonyms:  Watermelon mosaic virus 1, specific watermelon mosaic virus.
Group/Genus:  Potyviruses

Vector:  Aphids,  in non-persistent manner.
Transmission:  Mechanical
Main host plants:  Papaya, cucurbits.

Virus Particle:  Filamentous particles, containing RNA genome.
Virus Infection:
  Papaya ringspot virus (PRSV) infects papaya plants which show ring symptoms on fruit and distortion of leaves.  PRSV also occurs in cucurbit plants, and causes leaf mottling and distortion leaves and/or fruits. The virus is transmitted by aphids, and through mechanical contact among the plant tissues.   There is some report about seed transmission of PRSV.

Reference:
1.
CMI/AAB Descriptions of Plant Viruses, Nos. 63, 84, 292.


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